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Females in spawning process were not identified using macroscopic gross classification.

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They were measured, weighed and dissected, sex and maturation stage were analysed, first macroscopically, then part of the material was taken for microscopical confirmation.

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Young immature females were collected in June and from October till December, and mature ovaries were also present in different proportions during all the period, except in April.

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Sex plays an important role in the kinetics and dynamics of methylmercury in some animals.

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Values obtained graphically were analysed qualitatively due to the low sampling.

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A macroscopic analysis of the gonads permitted access to the sex and maturation stage, based on the classification proposed by Vazzolerwhich is:

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When grouped by trophic levels, overall mean difference in THg concentrations between males and females were not statistically significant.

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Values obtained graphically were analysed qualitatively due to the low sampling.

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